SPECIFIC STAINING OF SULPHATE GROUPS WITH ALCIAN BLUE AT LOW pH
نویسندگان
چکیده
منابع مشابه
Basophil counting with a new staining method using alcian blue.
Difficulties in obtaining reproducible and accurate enumeration of circulating basophils with existing techniques have hampered investigation of this infrequent cell population. A new basophil staining method is described that employs alcian blue dye for staining of heparin within basophils at low pH and in the presence of lanthanum ions. Basophil recognition is facilitated by reducing nonspeci...
متن کاملEffects of Oxidation upon Alcian Blue Staining of Acid Mucopolysaccharides.
Since Steedman ( 1950) has introduced it into histochemistry, alcian blue has been advocated to reveal a staining selectivity which depends upon the presence of acidic groups in mucopolysaccharides (Mowry, 1956; Pearse, 1960). As a plausible ground of this concept the fact has been presented that the alcian blue staining of polysaccharides can be increased in intensity, when acidic groups are i...
متن کاملTemperature-dependent staining reaction of chromatin by Alcian blue.
After treatment with Alcian blue at high temperatures, nuclei from chicken blood smears showed an intense staining reaction, which proved to be dependent on the DNA content of chromatin. The possibility that interactions between the phthalocyanine chromophore and unpaired bases accounts for this temperature-dependent staining of chromatin is briefly discussed.
متن کاملModified double skeletal staining protocols with Alizarin red and Alcian blue in laboratory animals
Abstract Purpose: Skeletal staining is a way to study the effect of many chemical or herbal substances on development of bones and cartilages in order to record the level of probable deformity.This article aimed to present a modified protocol to make all skeletal studies on laboratory animals possible. Materials and Methods: The important notes about skeletal staining were fixing by ethan...
متن کاملExperience with the sodium sulphate-Alcian Blue stain for amyloid in cardiac pathology.
The sodium sulphate-Alcian Blue (SAB) method, which stains amyloid green, was evaluated in 220 hearts from elderly patients. The technique proved superior to the Congo red, crystal violet, and thioflavine T methods used either singly or as a battery for the demonstration of cardiac amyloid. Amyloid was easily identified under the X3 objective, even in small amounts. A few non-amyloid components...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1964
ISSN: 0022-1554,1551-5044
DOI: 10.1177/12.4.309